FAST-TP53® NGS SEQUENCING KIT

FAST-TP53® SEQUENCING KIT is the NGS based kit developed to investigate mutations occurring in the TP53 gene in DNA molecules isolated from the blood or tissue. This kit, which was developed to sequence all exonic regions of the TP53 gene by the NGS method, achieves this with a single PCR reaction performed in 2 tubes.

USAGE AREAS

FAST-TP53® NGS SEQUENCING KIT was developed to enable the identification of molecular etiology in approximately 70% of cases of the LFS. Demonstration of genetic disorder in the Li-Fraumeni cases is extremely important in the diagnosis confirmation, genetic counselling, monitoring, and treatment.

ORDER PRODUCT

Description

TP53 Mutation Detection Kit with Next Generation Sequence Analysis

Li-Fraumeni syndrome (LFS) is a cancer susceptibility syndrome associated with an increased risk of developing soft tissue sarcoma, osteosarcoma, premenopausal breast cancer, brain tumours, adrenocortical carcinoma and leukemia. LFS related cancers often occur in childhood or young adulthood. Survivors of these cancers are at risk of contracting other cancers with advancing age.

Mutations in the TP53 gene can be identified in more than 70% of cases diagnosed with the clinical LFS according to clinical criteria and any history of cancer in the family. To date, no other gene has been identified that causes this syndrome. More than 95% of the responsible variants in this gene can be detected by sequence analysis of the coding regions of the TP53 gene.

ORDER PRODUCT

Additional information

TECHNICAL SPECIFICATIONS

Number of Tests 50 reaction
Transportation Conditions With dry ice and cold chain
Main Material DNA
Storage -20°C
Sequence Type NGS
Kit Content PCR Mix, Primer Mix-I, Primer Mix-II