FAST-JK2® SEQUENCING KIT

FAST-JK2 SEQUENCING KIT is a Sanger sequence analysis based kit developed to investigate mutations occurring in the JAK2 gene in the DNA molecules isolated from blood or tissue. This kit, which was developed to sequence 12th and 14th exons of the JAK2 gene by Sanger sequence analysis method, achieves this with a single PCR reaction that is realized in 5 tubes.

USAGE AREAS

FAST-JK2 SEQUENCING KIT, is used for the rapid, low-cost and reliable diagnosis of JAK2 gene 12th exon V167F mutation by a Sanger sequence analysis method. V167F mutation is most commonly observed mutation in JAK2 gene. This change in the aminoacid sequence causes uncontrolled blood cell production by constant expression of JAK2 protein. JAK2 mutations are mostly detected in patients diagnosed with Myeloproliferative Neoplasms (MPNs) such as Polycythemia Vera (PV) and Essential Thrombocythemia (ET).

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Description

Jak2 Mutation Detection With Sanger Sequencing

Janus Kinase 2 (JAK2) gene is responsible for production of JAK2 protein in cells. JAK2 tyrosine kinase protein is a specific protein of cytokine receptor signalling pathway. This receptor functionally interacts with prolactin receptor and is required for gamma interferon responses. JAK2 protein is involved in cell growth and it is especially important for the control of blood cell production in bone marrow.

This test allows for detection of bone marrow deficiencies. JAK2 related bone marrow deficiencies are known as Myeloproliferative Neoplasms (MPNs) which are characterized as excessive production of white blood cells, red blood cells and/or thrombocites in the bone marrow.

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Additional information

TECHNICAL SPECIFICATIONS

Number of Tests 50 reactions
Main Material DNA
Transportation Conditions With dry ice and cold chain
Storage -20°C
Sequenc Type Sanger
Kit Content JK2 SEQUENCING PCR MIX , JK2 SEQUENCING Exon 12 Forward Primer, JK2 SEQUENCING Exon 12 Reverse Primer, JK2 SEQUENCING Exon 14 Forward Primer, JK2 SEQUENCING Exon 14 Reverse Primer