FAST-FMF® NGS SEQUENCING KIT

FAST-FMF® NGS SEQUENCING KIT is a next generation sequence analysis based kit developed to investigate mutations occurring in the MEFV gene in the DNA molecules isolated from blood or tissue. This kit, which was developed to enrich all coding exons and exon-intron resultants of the MEFV gene for the next generation sequence analysis method, achieves this with a single PCR condition and a PCR reaction that is realized in a total of 2 tubes.

USAGE AREAS

FAST-FMF® NGS SEQUENCING KIT is used for the rapid, low-cost and reliable diagnosis of Familial Mediterranean Fever (FMF) by a next generation sequence analysis method. FMF is not only one of the most commonly occurring genetic diseases in our society, but it is also a very important disease in differential diagnosis of acute abdominal pain. It is imperative that patients with recurrent fever and attacks of pain be investigated.

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Description

Familial Mediterranean Fever (FMF) is an autosomal recessive inherited disease characterized by recurrent attacks of fever and pain. The disease follows a course with fever accompanied with peritonitis, pleuritis and arthritis. Typically, attacks accompanied by fever and serositis continue for 1 to 4 days with spontaneous healing being observed. Renal failure due to amyloidosis is the most serious complication of the disease. The disease is very common, especially in Turkish, Armenian, Arab and Jewish communities, as well as the Ashkenazi. In these communities, the incidence of the disease may increase to as much as one in 200 with a carrier frequency of one in five.

The disease is caused by mutations in the MEFV gene. The MEFV encodes the ‘pyrin’ protein. The MEFV encodes the broad ‘pyrin’ protein. The gene mainly manifests itself in granulocytes and is responsible for bringing inflammation under control. To date, up to 180 mutations have been identified in the MEFV gene.

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Additional information

TECHNICAL SPECIFICATIONS

Number of Tests 50 reactions
Transportation Conditions With dry ice and cold chain
Main Material DNA
Storage -20°C
Sequence Type NGS
Kit Content PCR Mix , Primer Mix-I, Primer Mix-II