FAST-CDH1® NGS SEQUENCING KIT

FAST-CDH1® NGS SEQUENCING KIT is the NGS based kit developed to investigate mutations occurring in the CDH1 gene within DNA molecules isolated from blood or tissue. This kit, which is developed for sequencing all exonic regions of the CDH1 gene by the NGS method, achieves this with a single PCR reaction performed in a single tube.

USAGE AREAS

FAST-CDH1® NGS SEQUENCING KIT was developed to enable the identification of molecular etiology in approximately 50 % of the HDGC and lobular breast cancer cases. Demonstration of genetic disorder in the HDGC and lobular breast cancer cases is extremely important when confirming the diagnosis, genetic counselling, monitoring, and treatment.

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Description

CDH1 Mutation Detection Kit with Next Generation Sequence Analysis

Hereditary diffuse gastric cancer (HDGC) is an autosomal dominant disease characterized by susceptibility to diffuse gastric cancer, which is a poorly differentiated adenocarcinoma that infiltrates in the form of increased gastric wall thickness without forming a significant mass. The average age of cancer development in the HDGC is 38 years and it occurs in individuals between 14-69 years of age. Mutations in the CDH1 gene are responsible for this susceptibility syndrome, and the cases among women who were diagnosed with mutations in this gene have a 39-52% risk of contracting lobular breast cancer, apart from the HDGC.

To date, the only gene identified to be responsible for the HDGK cases is CDH1, and mutation can be detected in this gene in about 50% of the cases. The pathogenic variant can be detected in more than 90% of the cases in which the mutation is detected, by sequence analysis in coding regions of the gene.

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Additional information

TECHNICAL SPECIFICATIONS

Number of Tests 25 reaction
Transportation Conditions With dry ice and cold chain
Main Material DNA
Storage -20°C
Sequence Type NGS
Kit Content PCR Mix, Primer Mix