FAST-BRCA® SEQUENCING KIT

FAST-BRCA® NGS SEQUENCING KIT is a next generation sequence analysis based kit developed to investigate mutations in the BRCA1 and BRCA2 genes in the DNA molecules isolated from blood or tissue. This kit, which was developed to enrich all the coding exons and exon-intron resultants of the BRCA1 and BRCA2 genes for the next generation sequence analysis method, achieves this with a single PCR (Polymerase chain reaction) condition and a PCR reaction that is realized in a total of 6 tubes.

USAGE AREAS

FAST-BRCA® NGS SEQUENCING KIT is used for the fast, low-cost and reliable diagnosis of hereditary breast-ovarian cancer syndrome (HBOC). Approximately 10% of all breast cancer cases are hereditary breast cancer. Mutations in BRCA1-BRCA2 genes need to be investigated, especially in breast cancer cases observed in women under 50 years of age and triple (-) breast cancer cases observed in those under 60 years of age.

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Description

The BRCA1 and BRCA2 genes are the most important tumor suppressor genes. The mutations occurring in these genes lead to familial cancer susceptibility patterns. This pattern inherited as autosomal dominant does not only predispose a person to breast cancer. BRCA1 and BRCA2-related hereditary breast and ovarian cancer syndromes are associated with increased risk for most commonly female and male breast cancer, ovarian cancer, and more rarely prostate cancer, pancreatic cancer, and malignant melanoma-like malignancies. In these genes, mutation-bearing cases carry a lifetime risk of malignancy of up to 80%. Its morbidity and mortality can be reduced through the preventive measures that can be taken after genetic diagnosis.

To date, more than 4,000 mutations have been identified in the BRCA1 and BRCA2 genes. These genes, which are somewhat difficult to analyse with the Sanger sequence analysis method, have become more workable with the use of next generation sequence analysis technology. Nevertheless, due to their size, investigating mutations in these genes is a significant problem.

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Additional information

TECHNICAL SPECIFICATIONS

Number of Tests 25 reactions
Transportation Conditions With dry ice and cold chain
Main Material DNA
Storage -20°C
Sequence Type NGS
Kit Content PCR Mix, Primer Mix-I, Primer Mix-II, Primer Mix-III, Primer Mix-IV, Primer Mix-V, Primer Mix-VI