FAST-APC® NGS SEQUENCING KIT

FAST-APC® NGS SEQUENCING KIT is the NGS based kit developed to investigate mutations occurring in the APC gene in DNA molecules isolated from blood or tissue. This kit, which is developed to sequence all exonic regions of the APC gene by the NGS method, achieves this with a single PCR reaction performed in 2 tubes.

USAGE AREAS

FAST-APC® NGS SEQUENCING KIT was developed to enable the identification of molecular etiology in more than 95% of the FAP cases. In addition, this kit can detect mutations in the APC gene in the cases of the “atenue FAP” and the “GAPPS”. Demonstration of genetic disorder in the FAP cases is extremely important in the diagnosis confirmation, genetic counseling, monitoring, and treatment.

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Description

APC Mutation Detection Kit with Next Generation Sequence Analysis

Familial adenomatous polyposis (FAP) is a colon cancer predisposition syndrome characterized by the development of thousands of adenomatous colonic polyps beginning at an average age of 16 years (7-36 years). Untreated patients develop colon cancer at an average age of 39 years (34-43 years).

A definite diagnosis of the FAP cases, which are clinically diagnosed with characteristic symptoms and findings, can be established by determining the heterozygous pathogenic variant in the APC gene.

The mutations in the APC gene are also responsible for the “atenue FAP” and “GAPPS: Gastric Adenocarcinoma and Proximal Polyposis of the Stomach” clinical manifestations, other than the FAP.

In the genetic diagnosis of the FAP disease, a responsible mutation can be detected in about 90% of the cases by sequence analysis of the coding regions of the APC gene.

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Additional information

TECHNICAL SPECIFICATIONS

Number of Tests 25 reaction
Transportation Conditions With dry ice and cold chain
Main Material DNA
Storage -20°C
Sequence Type NGS
Kit Content PCR Mix, Primer Mix-I, Primer Mix-II